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Microbial Institute for Fermentation Industry

R&D기술자원

한국형유용균주산업화기반구축

SJFI 새로운 발효의 탄생 리던

논문

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    보유기술논문 - 글조회
    논문제목 구연산 처리에 의한 고욤나무 잎 Myricitrin의 Myricetin으로 전환 최적화
    저자명 강현진, 오현화, 송근섭, 장선일, 김영수
    첨부파일 목록
      구연산 처리에 의한 고욤나무 잎 Myricitrin의 Myricetin으로 전환 최적화.pdf [869913 byte]
    등재학술지명 한국식품영양과학회
    페이지 50(5), 483-490
    주요내용

    Citric acid treatment conditions for converting Diospyros lotus leaves to myricetin were optimized using the response surface methodology. The contents of myricitrin and myricetin in extracts of Diospyros lotus leaves were measured using high performance liquid chromatography (HPLC). From several organic acids screened, citric acid produced the highest content of myricetin, and was therefore selected for the conversion of myricitrin from Diospyros lotus leaf extracts. The treatment method of adding citric acid was optimized by the response surface methodology (RSM) using the Box-Behnken design (BBD) with three variables: molar concentration of citric acid (X 1 : 50∼150 mM), reaction temperature (X 2 : 85∼95°C) and reaction time (X 3 : 3∼7 h). RSM analysis revealed that the content of myricitrin and myricetin was highly impacted by the citric acid concentration, whereas reaction temperature had the lowest effect. To verify accuracy of the prediction, the myricetin content was determined under optimal conditions, and the result obtained (1,224.99 mg/100 g DLP) was similar to those predicted. Based on these results, we propose the use of citric acid treatment to increase the yield of myricetin from Diospyros lotus leaves.